iris elispot plate reader  (Mabtech Inc)

Journal: Microorganisms

Article Title: Immunogenicity Analysis and Identification of Potential T-Cell Epitopes in C129R Protein of African Swine Fever Virus

doi: 10.3390/microorganisms12061056

Figure Lengend Snippet: IFN-γ response of splenic lymphocytes from mice immunized with C129R protein. Splenic lymphocytes from mice vaccinated with the C129R protein were collected and stimulated with C129R protein for in vitro restimulation for IFN-γ ELISpot. ( a ) Results of the assay for IFN-γ in immunized mice are presented as SFC/10 5 cells, with the mean stimulated spots in each group presented as a bar graph and the error bars representing the standard deviation. Statistically significant differences are indicated by asterisks. ** p < 0.01; *** p < 0.001. ( b ) ELISpot Spot Plot. 1#–3# indicates 3 representative mice.

Article Snippet: After the plates were completely dry, spot-forming units (SFU) were counted on an IRIS Mabtech ELISpot plate reader.

Techniques: In Vitro, Enzyme-linked Immunospot, Standard Deviation

Journal: Microorganisms

Article Title: Immunogenicity Analysis and Identification of Potential T-Cell Epitopes in C129R Protein of African Swine Fever Virus

doi: 10.3390/microorganisms12061056

Figure Lengend Snippet: Four T-cell epitopes were identified on the C129R protein. IFN-γ responses were detected using ELISpot in the splenic lymphocytes of mice inoculated with C129R protein. ( a ) ELISpot spot graph (only one representative result is shown). ( b ) ELISpot bar chart. IFN-γ responses were detected in the splenic lymphocytes of mice inoculated with C129R protein using ELISpot. *** p < 0.001.

Article Snippet: After the plates were completely dry, spot-forming units (SFU) were counted on an IRIS Mabtech ELISpot plate reader.

Techniques: Enzyme-linked Immunospot